Hōʻike ʻia ʻo yest Cell Protein
ʻO ka ʻōnaehana hōʻike hū he ala i hoʻohana nui ʻia no ka hōʻike ʻana i ka protein eukaryotic, ma muli o kona maʻalahi i ka mahiʻai ʻana, affordability, a me ka maʻalahi o ka hana.Ma waena o nā ʻano hū like ʻole, ʻo Pichia pastoris ka mea hoʻokipa kaulana loa, no ka mea e hoʻomaʻamaʻa i ka ʻōlelo protein intracellular a me extracellular.Hiki i ka ʻōnaehana ke hoʻololi ma hope o ka unuhi ʻana, e like me ka phosphorylation a me ka glycosylation, ka hopena i kahi ʻōnaehana hōʻike eukaryotic kūʻokoʻa me nā pono he nui.
Nā mea lawelawe | Lead Time(BD) |
ʻO ka hoʻonui ʻana i ka Codon, ka hoʻohui ʻana o ka gene a me ka subcloning | 5-10 |
ʻO ka nānā ʻana i ka clone maikaʻi | 10-15 |
Hōʻike liʻiliʻi | |
ʻO ka hōʻike a me ka hoʻomaʻemaʻe ʻana i ka nui (200ML), nā mea hoʻopuka e komo pū me ka protein i hoʻomaʻemaʻe ʻia a me ka hōʻike hoʻokolohua |
Inā hoʻokomo ʻia ka gene i Bioantibody, e hoʻokomo ʻia ka plasmid i kūkulu ʻia i loko o nā mea lawe.