Hōʻike Protein Cell Insect
He ʻōnaehana hōʻike eukaryotic i hoʻohana mau ʻia ka ʻōnaehana hōʻike cell insect no ka hōʻike ʻana i nā protein nui-molecular.Ke hoʻohālikelike ʻia me nā pūnana mammalian, maʻalahi nā kūlana moʻomeheu cell insect a ʻaʻole koi iā CO2.ʻO Baculovirus kahi ʻano o ka maʻi maʻi DNA kaulua me nā pūnae pepeke ma ke ʻano he mea hoʻokipa maoli.He kiʻekiʻe kona ʻano kikoʻī, ʻaʻole e hoʻopilikia i nā vertebrates, a ʻaʻohe pōʻino i ke kanaka a me nā holoholona.ʻO sf9, ka mea maʻamau i hoʻohana ʻia ma ke ʻano he pūnaewele hoʻokipa, ʻike ʻia i loko o ka planktonic a i ʻole pili i ka moʻomeheu.He kūpono loa ka sf9 no ka hōʻike nui, a hiki ke hoʻohana ʻia no ka hana ʻana a me ka hoʻololi ʻana o nā protein e like me ka phosphorylation, glycosylation, a me ka acylation.Hiki ke hoʻohana ʻia ka ʻōnaehana hōʻike insect cell no ka hōʻike ʻana i nā genes he nui, a hiki ke hōʻike pū i nā protein ʻona e like me nā peptides antimicrobial.
| Nā mea lawelawe | Lead Time（BD） |
| ʻO ka hoʻonui ʻana i ka Codon, ka hoʻohui ʻana o ka gene a me ka subcloning | 5-10 |
| P1 generation virus incubation and small scale expression | 10-15 |
| P2 generation virus incubation, hōʻike nui a me ka hoʻomaʻemaʻe, ka lawe ʻana i ka protein i hoʻomaʻemaʻe ʻia a me ka hōʻike hoʻokolohua |