Hoʻohana ʻia:
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (Immunochromatographic Assay) he in vitro, rapid, lateral flow test, i ʻike ʻia ma ke ʻano he lateral flow immunochromatographic assay, i manaʻo ʻia no ka ʻike qualitative o S. Typhi a me Paratyphi antigens i loko o nā fecal specimens mai. nā maʻi.Pono e unuhi ʻia nā hopena mai ka S. Typhi/Paratyphi Combo Antigen Rapid Test Kit me ka loiloi lāʻau lapaʻau o ka mea maʻi a me nā ʻano hana diagnostic ʻē aʻe.
Nā Kumu Hoʻāʻo:
ʻO S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (Immunochromatographic Assay) he lateral flow chromatographic immunoassay.Loaʻa iā ia ʻekolu mau laina pre-coated, "T1" S. Typhi Test line, "T2" Paratyphi Test line a me "C" Control line ma ka membrane nitrocellulose.ʻIole monoclonal anti-S.Hoʻopili ʻia nā antibodies Typhi a me anti-Paratyphi ma ka ʻāpana laina hoʻāʻo a hoʻopaʻa ʻia nā antigens anti-chicken IgY Goat ma ka ʻāpana hoʻomalu. ka S. Typhi/Paratyphi Antibody-labeled conjugate e hoʻokumu ana i nā mea ʻāpana kala antigen-antibody.E neʻe ana nā complexes ma ka membrane nitrocellulose ma o ka hana capillary a hiki i ka laina hoʻāʻo, kahi i hopu ʻia ai e ka ʻiole monoclonal anti-S.ʻO nā antibodies Typhi/Paratyphi.ʻIke ʻia kahi laina T1 kala i ka puka aniani hopena inā loaʻa nā antigens S. Typhi i ka specimen a pili ka ikaika i ka nui o ka antigen S. Typhi.ʻIke ʻia kahi laina T2 kala ma ka puka aniani hopena inā loaʻa nā antigens Paratyphi i ka specimen a pili ka ikaika i ka nui o ka antigen Paratyphi.Inā ʻaʻole i loaʻa nā antigens S.Typhi/Paratyphi i loko o ka specimen a i ʻole ma lalo o ka palena ʻike, ʻaʻole ʻike ʻia kahi pūʻulu kala i ka laina hoʻāʻo (T1 a me T2) o ka hāmeʻa.Hōʻike kēia i kahi hopena maikaʻi ʻole.ʻAʻole ʻike ʻia ka laina hoʻāʻo a i ʻole ka laina mana ma ka puka aniani hopena ma mua o ka hoʻohana ʻana i ka specimen.Pono ka laina mana ʻike e hōʻike i ka pololei o ka hopena
Mahele REF REF | B033C-01 | B033C-05 | B033C-25 |
Ka Pepa Hoao | 1 hoao | 5 hoao | 25 hoao |
Hoʻopaʻa | 1 omole | 5 omole | 25/2 omole |
ʻEke ʻAi Hoʻohālike | 1 apana | 5 pcs | 25 pcs |
Nā kuhikuhi no ka hoʻohana ʻana | 1 apana | 5 pcs | 25 pcs |
Palapala Kulike | 1 apana | 1 apana | 1 apana |
KaʻAnuʻu 1: Laʻanae Hoʻomākaukau
1. E hōʻiliʻili i nā ʻano fecal i loko o nā pahu maʻemaʻe a me ka leak-proof.
2. Ka lawe ʻana a me ka mālama ʻana i nā mea hoʻohālike: Hiki ke mālama ʻia nā mea hoʻohālike ma ka lumi wela no 8 mau hola a i ʻole i loko o ka pahu hau ma 36°F a 46°F (2°C a 8°C) a hiki i 96 hola.
3. Hiki ke hoʻoheheʻe ʻia nā mea hoʻohālike fecal i mālama ʻia i ka hau a hiki i 2 mau manawa ma -10°C a i ʻole.Inā hoʻohana ʻoe i nā specimens hau, e hoʻoheheʻe i ka lumi wela.Mai ʻae i nā ʻano fecal e noho i loko o ka hui hoʻoheheʻe no >2 mau hola.
KaʻAnuʻu 2: Hoʻāʻo
1. E ʻoluʻolu e heluhelu pono i nā ʻōlelo aʻo ma mua o ka hoʻāʻo ʻana.Ma mua o ka hoʻāʻo ʻana, e ʻae i nā cassettes hoʻāʻo, ka hāpana hāpana a me nā laʻana e kaulike i ka wela lumi (15-30 ℃ a i ʻole 59-86 degere Fahrenheit).
2. Wehe i kahi cassette ho'āʻo mai ka ʻeke pepa a kau ma kahi pālahalaha.
3. Wehe i ka hue laʻana, e hoʻohana i ka lāʻau applicator i hoʻopaʻa ʻia ma ka pāpale e hoʻololi i kahi ʻāpana liʻiliʻi o ka pahu (3- 5 mm ke anawaena; ma kahi o 30-50 mg) i loko o ka ʻōmole hāpana i loko o ka pahu hoʻomākaukau specimen.
4. Hoʻopili i ka lāʻau i loko o ka ʻōmole a hoʻopaʻa paʻa.E hoʻohui i ka laʻana o ka pahu me ka mea hoʻopaʻa ma ka lulu ʻana i ka ʻōmole no nā manawa he nui a waiho i ka paipu no 2 mau minuke.
5. Wehe i ka piko o ka omole la'ana a pa'a i ka omole ma ke kulana ku pono ma luna o ka luawai la'ana o ka Cassette, e ho'opuka i 3 kulu (100 -120μL) o ka la'ana wai i hooheheeia i ka luawai.
KaʻAnuʻu 3: Heluhelu
E heluhelu i nā hopena i loko o 15-20 mau minuke.ʻAʻole ʻoi aku ka manawa wehewehe o ka hopena ma mua o 20 mau minuke
1. S. Typhi Ka hopena
Hōʻike ʻia nā kaha kala ma ka laina hoʻāʻo (T1) a me ka laina hoʻomalu (C).Hōʻike ia i ka hopena maikaʻi no nā antigens S. Typhi i ka specimen.
2. Ka hopena maikaʻi o Paratyphi
Hōʻike ʻia nā kaha kala ma ka laina hoʻāʻo (T2) a me ka laina hoʻomalu (C).Hōʻike ia i ka hopena maikaʻi no nā antigens Paratyphi i ka specimen.
3. S. Typhi a me Paratyphi hopena maikaʻi
Hōʻike ʻia nā kaha kala ma ka laina hoʻāʻo ʻelua (T1), laina hoʻāʻo (T2) a me ka laina hoʻokele (C).Hōʻike ia i ka hopena maikaʻi no nā antigens S. Typhi a me Paratyphi i ka specimen.
4. Ka hopena maikaʻi ʻole
Hōʻike ʻia ka pūʻulu kala ma ka laina mana (C) wale nō.Hōʻike ia ʻaʻole i loaʻa ka ʻike o nā antigens S. Typhi a i ʻole Paratyphi a ma lalo o ka palena ʻike o ka hoʻāʻo.
5. Ka hopena kūpono ʻole
ʻAʻohe pūʻulu kala ʻike ʻia ma ka laina hoʻomalu ma hope o ka hana ʻana i ka hoʻāʻo.ʻAʻole paha i hahai pono ʻia nā kuhikuhi a i ʻole ua pōʻino paha ka hoʻāʻo.Manaʻo ʻia e hoʻāʻo hou i ka specimen
inoa mea kūʻai | Cat.ʻAʻole | Nui | Hoʻohālike | Ola Pahu | ʻO Trans.& Sto.Temp. |
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit(Immunochromatographic Assay) | B033C-01 | 1 ho'āʻo / pahu | Fecal | 24 mahina | 2-30 ℃ |
B033C-05 | 5 ho'āʻo/kit | ||||
B033C-25 | 25 ho'āʻo/kit |