Hoohana Manao
Hoʻolālā ʻia ʻo Malaria antigen detection kit ma ke ʻano maʻalahi, wikiwiki, qualitative a me ke kumu kūʻai kūpono no ka ʻike like ʻana a me ka hoʻokaʻawale ʻana o Plasmodium falciparum (Pf) a me Plasmodium vivax (Pv) i ke koko holoʻokoʻa o ke kanaka a i ʻole ka manamana lima.Hoʻohana ʻia kēia mea hana ma ke ʻano he hōʻike hōʻike a hoʻohana ʻia no ka hōʻoia kōkua o ka maʻi P. f a me Pv.
Kumu Hoao
Hoʻokumu ʻia ka pahu hōʻike hōʻike ʻo Malaria antigen (Lateral chromatography) ma ke kumu o ka microsphere double antibody sandwich method i ka wikiwiki qualitative hoʻoholo o Pf/Pv antigen i ke koko holoʻokoʻa kanaka a i ʻole ka manamana lima.Hōʻailona ʻia ka microsphere i ka anti-HRP-2 antibody (specific to Pf) ma ka T1 band a me ka anti-PLDH antibody (specific to Pv) ma ka T2 band, a ua uhi ʻia ka anti-mouse IgG polyclonal antibody ma luna o ka wahi mana maikaʻi (C ).Ke loaʻa i ka hāpana ka maʻi malaria HRP2 a i ʻole pLDH antigen a ʻoi aku ka kiʻekiʻe ma mua o ka palena ʻike liʻiliʻi, i ʻae ʻia e hana me ka microsphere colloidal i uhi ʻia me Mal-antibody e hana i ka paʻakikī antibody-antigen.A laila neʻe ka paʻakikī ma ka ʻaoʻao o ka membrane a hoʻopaʻa ʻia i ka antibody immobilized ma ka membrane e hana ana i kahi laina ʻulaʻula ma ka ʻāpana hoʻāʻo, e hōʻike ana i kahi hopena maikaʻi.ʻO ka hiki ʻana mai o ka laina hoʻomalu e hōʻike ana ua hana pololei ʻia ka hoʻāʻo me ka nānā ʻole ʻana o ka antigen Pf/Pv.
ComponentREF | B013C-01 | B013C-25 |
Ka Pepa Hoao | 1 hoao | 25 hoao |
Laʻana Diluent | 1 omole | 1 omole |
Pahu wai | 1 apana | 25 PCS |
Nā kuhikuhi no ka hoʻohana ʻana | 1 apana | 1 apana |
Palapala Kulike | 1 apana | 1 apana |
E ʻohi pono i ke koko holoʻokoʻa o ke kanaka a i ʻole ke koko manamana lima.
1. Wehe i kahi paipu unuhi mai ka kit a me ka pahu ho'āʻo mai ka ʻeke kiʻiʻoniʻoni ma ka haehae ʻana i ka notch.E kau ma luna o ka mokulele ākea.
2. E wehe i ka ʻeke pepa alumini pepa nānā.Wehe i ke kāleka hoʻāʻo a kau ʻia ma ka pākaukau.
3. E hoʻohui koke i 60μL hāpana dilution solution.E hoʻomaka i ka helu.
Ma hope o 20 mau minuke, e heluhelu i nā hopena.(E hoʻomaopopo: mai heluhelu i nā hopena ma hope o 30 mau minuke!)
1.Pf Pono
ʻO ka loaʻa ʻana o ʻelua mau kala kala ("T1" a me "C") i loko o ka puka aniani hopena e hōʻike ana iā Pf Positive.
2.Pv Pono
ʻO ka loaʻa ʻana o ʻelua mau kala kala ("T2" a me "C") i loko o ka puka aniani hopena e hōʻike ana iā Pv
3.Poitive.Pf a me Pv Pono
ʻO ka loaʻa ʻana o ʻekolu mau kala kala ("T1", "T2" a me "C") i loko o ka puka aniani hopena e hōʻike ana i ka maʻi hui pū ʻana o P. f a me Pan.
4.Negative Hua
ʻO ka loaʻa ʻana o ka laina hoʻomalu (C) wale nō i loko o ka puka makani hopena e hōʻike ana i kahi hopena maikaʻi ʻole.
5. Ka hopena kūpono ʻole
Inā ʻaʻole ʻike ʻia kahi hui ma ka ʻāpana hoʻomalu (C), ʻaʻole kūpono nā hopena hōʻike me ka nānā ʻole ʻana a i ʻole ka laina ma ka ʻāpana hoʻāʻo (T).ʻAʻole i hahai pololei ʻia ke kuhikuhi a i ʻole ua pōʻino paha ka hoʻāʻo.
inoa mea kūʻai | Cat.ʻAʻole | Nui | Hoʻohālike | Ola Pahu | ʻO Trans.& Sto.Temp. |
ʻO Malaria HRP2/pLDH (Pf/Pv) ʻAtigen Rapid Test Kit (Lateral chromatography) | B013C-01 | 1 ho'āʻo / pahu | Koko holoʻokoʻa/Koko manamanalima | 18 mahina | 2-30 ℃ / 36-86 ℉ |
B013C-25 | 25 ho'āʻo/kit |