Hoohana Manao
He kūpono kēia huahana no ka hoʻomaʻamaʻa ʻana o ka serum/plasma/holo blood samples no ka ʻike ʻana i nā antibodies e kūʻē iā Leishmania.He ho'āʻo maʻalahi, wikiwiki a ʻaʻohe mea kani no ka ʻike o kala-azar i hana ʻia e Leishmania.
Kumu Hoao
ʻO kēia huahana he lateral flow chromatographic immunoassay.Aia i loko o ka pahu ho'ā'o: 1) kahi pad conjugate kala burgundy i loa'a ka antigen rK39 recombinant i hui pū me ke gula colloid (Leishmania conjugates) a me nā conjugates IgG-gula rabbit;2) kahi ʻāpana membrane nitrocellulose i loaʻa i ʻelua mau pahu hoʻāʻo (M a me G band) a me kahi pūʻali mana (C band).
Nā lako / hāʻawi ʻia | Nui | Ka nui(5 ho'āʻo/Kit)
| Nui
|
Kiko hoao | 1 hoao | 5 hoao | 25 hoao |
Hoʻopaʻa | 1 omole | 5 omole | 25/2 omole |
Pahu wai | 1 apana | 5 pcs | 25 pcs |
ʻEke ʻAi Hoʻohālike | 1 apana | 5 pcs | 25 pcs |
Lancet hiki ke hoopau | 1 apana | 5 pcs | 25 pcs |
Nā kuhikuhi no ka hoʻohana ʻana | 1 apana | 1 apana | 1 apana |
Palapala Kulike | 1 apana | 1 apana | 1 apana |
E hōʻiliʻili pono i ke koko kanaka / Plasma / Whole koko.
E ʻoluʻolu e heluhelu pono i nā kuhikuhi ma mua o ka hoʻāʻo ʻana.Ma mua o ka hoʻāʻo ʻana, e ʻae i ka pahu hoʻāʻo, ka hāpana hāpana a me ka laʻana e kaulike i ka mahana (15-30 ℃ a i ʻole 59-86 degere Fahrenheit).
1. Wehe i kahi paipu unuhi mai ka pahu a me kahi pahu hoʻāʻo mai ka ʻeke kiʻiʻoniʻoni ma ka haehae ʻana i ka notch.E kau iā lākou ma ka mokulele ākea.
2. Wehe i ka ʻeke pepa alumini pepa nānā.Wehe i ke kāleka hoʻāʻo a kau ʻia ma ka pākaukau.
3.E hoʻohana i ka pipette hoʻokuʻu, e hoʻololi i hoʻokahi kulu (e pili ana i 20μL) fingertip koko / a i ʻole 4μL serum / a i ʻole 4μL plasma / a i ʻole 4μL koko holoʻokoʻa i loko o ka hāpana maikaʻi ma ka cassette hōʻike.
4.Wehe i ka paipu pale.E hoʻokomo i 3 kulu (e pili ana i 80 μL) o ka mea hoʻoheheʻe assay i loko o ka mea hoʻoheheʻe assay me ke ʻano poepoe.E hoʻomaka i ka helu.
E heluhelu i ka hopena ma 5-10 min.ʻAʻole kūpono nā hopena ma hope o 10 mau minuke.
Ka hopena maikaʻi ʻole
Inā ʻike wale ʻia ka laina hoʻomalu maikaʻi C a ʻaʻole hōʻike nā laina ʻike G a me M
Ka hopena maikaʻi
1. ʻO ka laina hoʻomalu maikaʻi C a me ka laina ʻike M ua ʻike ʻia = ʻike ʻia ka antibody Leishmania IgM, a maikaʻi ka hopena no ka antibody IgM.
2. ʻIke ʻia ka laina hoʻokele maikaʻi C a me ka laina ʻike G = ʻike ʻia ka antibody Leishmania IgG a maikaʻi ka hopena no ka antibody IgG.
3. Hōʻike ʻia ka laina hoʻokele maikaʻi C a me nā laina ʻike G a me M = nā antibodies Leishmania IgG a me IgM.ʻike ʻia, a maikaʻi ka hopena no nā antibodies IgG a me IgM.
Ka hopena kūpono ʻole
ʻAʻole hiki ke nānā ʻia ka laina hoʻokele maikaʻi C, ʻaʻole kūpono nā hopena inā ʻaʻole e hōʻike ʻia kahi laina hoʻāʻo, a pono e hana hou ʻia ka hōʻike.
inoa mea kūʻai | Cat.ʻAʻole | Nui | Hoʻohālike | Ola Pahu | ʻO Trans.& Sto.Temp. |
Leishmania IgG/IgM Antibody Test Kit (Immunochromatographic Assay) | B020C-01 | 1 ho'āʻo / pahu | Serum / Plasma / Koko holoʻokoʻa | 18 mahina | 2-30 ℃ / 36-86 ℉ |
B020C-05 | 5 ho'āʻo/kit | ||||
B020C-25 | 25 ho'āʻo/kit |