ʻOihana Antibody Monoclonal
ʻO ka antibody monoclonal, i hana ʻia e kahi clone cell B hoʻokahi, he homogeneity kiʻekiʻe e kuhikuhi ana i kahi epitope antigen kikoʻī.He nui nā pōmaikaʻi o ka antibody monoclonal, me ka maʻemaʻe kiʻekiʻe, ka naʻau, a me ka kikoʻī.Hoʻokumu ʻia nā Hybridoma ma o ka hui ʻana o nā lymphocytes B e hoʻopuka ana i kekahi mau antibodies kikoʻī, me nā cell myeloma lōʻihi.Hoʻohana ʻo Bioantibody i kahi ʻenehana fusion kiʻekiʻe i ʻoi aku ka maikaʻi o 20 mau manawa ma mua o nā ʻano fusion maʻamau.Hoʻohui ʻia, hoʻohana ia i ka ʻenehana screening microarray protein e ʻike ai i nā antibodies monoclonal e hōʻike ana i ka kikoʻī kiʻekiʻe, pili, a me ka pono hana e kūʻē i nā epitopes kikoʻī.
Nā mea lawelawe | Nā mea hoʻokolohua | Lead Time(Week) |
Hoʻomākaukau antigen | 1. Hāʻawi ka mea kūʻai aku i ka antigen2. Hoʻomākaukau ka bioantibody i ka antigen | / |
Kaʻiole ʻiole | ʻO ka pale ʻana o ka ʻiole BALB/c, ka hōʻiliʻili serum a me ka nānā ʻana ELISA | 4 |
ʻO ka hui pū ʻana a me ka nānā ʻana | ʻO ka hui ʻana o nā splenocytes ʻiole a me nā cell myeloma, ka nānā ʻana i ka HAT | 2 |
Hoʻokumu laina kelepona paʻa | Hoʻokaʻawale ʻia nā clones maikaʻi i hoʻopaʻa ʻia | 3 |
ʻIke ʻana i ka antibody isotype | Ka ʻike ʻana i nā ʻano subtype laina kelepona | 1 |
ʻO ka hoʻoulu ʻana i ka pānaʻi liʻiliʻi | ʻO ka hoʻoulu ʻana i ka serum-free | 2 |
ʻO ka incubation nui a me ka hoʻomaʻemaʻe | 200mL serum-free incubation a hoʻomaʻemaʻe | 1 |