Hoohana Manao
Kūpono ʻo SARS-CoV-2 Neutralizing antibody Test Kit (Lateral chromatography) no ka in vitro qualitative ka ʻike wikiwiki ʻana o SARS-CoV-2 neutralizing antibodies i loko o ke kanaka serum, plasma, a i ʻole nā koko koko holoʻokoʻa (capillary a i ʻole venous).Hoʻolālā ʻia ka pahu i mea kōkua e loiloi i ka pane ʻana o ka immune adaptive i ka SARS-CoV-2.No ka hoʻohana diagnostic in vitro wale nō.No ka hoohana oihana wale no.
Kumu Hoao
ʻO SARS-CoV-2 Neutralizing antibody Test Kit (Lateral chromatography) kahi immunoassay e pili ana i ka membrane qualitatively no ka ʻike ʻana i nā antibodies SARS-CoV-2 RBD i ka serum, plasma a me ke koko holoʻokoʻa.Hoʻokuʻu ʻia ka hāpana i loko o ka pūnāwai hāpana a hoʻohui ʻia ka mea hoʻoheheʻe dilution buffer ma hope.ʻO nā antibodies SARS-CoV-2 RBD i loko o ka laʻana e hui pū me ka protein RBD i hoʻopaʻa inoa ʻia a hana i nā paʻakikī immune.I ka neʻe ʻana o ka paʻakikī ma ka membrane nitrocellulose e ka hana capillary, hiki ke hopu ʻia nā antibodies RBD e kekahi protein RBD ʻē aʻe i uhi ʻia ma ka wahi hoʻāʻo (laina T), e hana i kahi laina hōʻailona.Hoʻopili ʻia ka wahi hoʻomalu maikaʻi me ka IgY anti-chicken kao, a hopu ʻia ka moa i hoʻopaʻa inoa ʻia ʻo IgY e hana i kahi paʻakikī a hōʻuluʻulu i ka laina C.Inā ʻaʻole ʻike ʻia ka laina C, hōʻike ia he hewa ʻole ka hopena, a pono e hoʻāʻo hou.
Māhele/REF | B006C-01 | B006C-25 |
Ka Pepa Hoao | 1 hoao | 25 hoao |
Papa Waiona | 1 apana | 25 pcs |
Laʻana Diluent | 1 omole | 25 omole |
Nā kuhikuhi no ka hoʻohana ʻana | 1 apana | 1 apana |
Lancet hiki ke hoopau | 1 apana | 25 PCS |
Pāpaʻu | 1 apana | 25 PCS |
Palapala Kulike | 1 apana | 1 apana |
KaʻAnuʻu 1: Sampling
E hōʻiliʻili pono i ke koko kanaka / Plasma / Whole koko.
KaʻAnuʻu 2: Hoʻāʻo
1. E wehe i ka ʻeke pepa alumini pepa nānā.Wehe i ke kāleka hoʻāʻo a kau ʻia ma ka pākaukau.
2. E hoʻohana i ka pipette hoʻokuʻu, e hoʻoili i ka 10µL serum / a i ʻole 10µL plasma/ a i ʻole 20µL koko holoʻokoʻa i loko o ka pūnāwai maikaʻi ma ka cassette hoʻāʻo.
3. E wehe i ka paipu pale ma ka wili ʻana i luna.E hoʻopaʻa pololei i ka ʻōmole hoʻopaʻa a 1 knm ma luna o ka pūnāwai.E hoʻohui i ʻekolu kulu (e pili ana i 100 µL) o ka mea hoʻopaʻa i loko o ka pahu paʻa pono ma ka pahu hoʻāʻo.
KaʻAnuʻu 3: Heluhelu
Ma hope o 10 mau minuke, e heluhelu i nā hopena.(Manuahi: hanaAOLEe heluhelu i nā hopena ma hope o 15 mau minuke!)
Ka hopena maikaʻi
Inā ʻike ʻia ka laina C mana maikaʻi a me ka laina ʻike T, ʻo ia ka mea ua ʻike ʻia ka SARS-CoV-2 neutralizing antibodies, a maikaʻi ka hopena no ka neutralizing antibodies.
Ka hopena ʻino
Inā ʻike wale ʻia ka laina C control maikaʻi a ʻaʻole hōʻike ka laina ʻike T i ke kala, ʻo ia ka mea ʻaʻole i ʻike ʻia ka SARS-CoV-2 neutralizing antibodies a ʻaʻole maikaʻi ka hopena.
Ka hopena kūpono ʻole
Inā ʻaʻole hiki ke nānā ʻia ka laina C mana maikaʻi, ʻaʻole kūpono ka hopena me ka nānā ʻole inā aia kahi hōʻike laina ʻike, a pono e hana hou ʻia ka hoʻāʻo.
inoa mea kūʻai | Cat.ʻAʻole | Nui | Hoʻohālike | Ola Pahu | ʻO Trans.& Sto.Temp. |
ʻO SARS-CoV-2 ka pahu hoʻāʻo antibody neutralizing (Lateral chromatography) | B006C-01 | 1 ho'āʻo / pahu | S/P/WB | 18 mahina | 2-30 ℃ / 36-86 ℉ |
B006C-25 | 25 ho'āʻo/kit |